ABSTRACT
Background: Diabetes mellitus is a major endocrine disorder and growing health problem in most countries. We studied effects of Avicennia marina fruits aqueous and hydroalcoholic extract in the diabetic rat by streptozotocin
Materials and methods: In this experimental study, 45 male rats were divided into 9 groups of 5, including sham group, diabetes sham, positive sham [diabetes + 0.9% normal saline] and treated diabetic groups orally with aqueous and hydroalcoholic extract of Avicennia marina fruits [30, 60, 120 mg/kg daily for three consecutive days]. Twenty-four hours after the last treatment rats, blood samples were collected from heart for determining the blood glucose and insulin concentration levels using glucose oxidase and enzyme-linked immunosorbent assay [ELISA] methods respectively
Results: All doses of Avicennia marina fruits aqueous and hydroalcoholic extract significantly decreased serum glucose level of diabetic test rats compared to treated diabetic sham rats. 60mg/kg of Avicennia marina fruits hydroalcoholic extract and all doses of Avicennia marina fruits aqueous extract significantly increased serum insulin level of diabetic test rats compared to treated diabetic sham rats
Conclusion: In this study, we have shown that Avicennia marina fruits aqueous and hydroalcoholic extract can significantly decrease the blood glucose
ABSTRACT
Reactive oxygen species and oxidative stress impair beta-cell function and reduce insulin secretion. It has been shown that progesterone and cilostazol possess antioxidant properties. The present study was aimed to investigate in vitro pretreatment effect of progesterone and cilostazol on insulin secretion as well as their protective effects against hydrogen peroxide-induced oxidative stress in pancreatic isolated islets from mouse. Pancreatic islets were isolated from 84 male NMRI mice [25-30g] by collagenase digestion method and pretreated for 48h with cilostazol [10 microM], progesterone [0.5 microM] and glibenclamide [10 microM] in culture medium. Then islets were exposed to hydrogen peroxide [H2O2. 500 microM] for 2h. Next, culture mediums containing glucose concentration of 2.8 mM or 16.7 mM were added to them and incubated in this status for 1h. At the end, the rate of insulin output from islets, lipid peroxidation and antioxidant enzymes activities in islet tissues were assayed. Exposure of islets to H2O2, resulted in a significant decrease in insulin secretion, superoxide dismutase and catalase activities [P<0.001]. Also islets malondialdehyde levels were increased by H2O2, after addition of 2.8mM [P<0.05] and 16.7mM [P<0.001] glucose. 48h pretreatment of islets with cilostazol and progesterone, significantly reverted back this changes [P<0.05]. Results of present study showed that cilostazol and progesterone protect mice pancreatic islets against H2O2-induced oxidative stress. At the end, our results suggested that protective effects of progesterone and cilostazol are mediated by augmentation the antioxidant defence system of islets